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Consider a two-photon excitation process where the wavenumber of the excitation light is 10000cm1. Assume an internal conversion of 6.891020J. If the fluorescence from both
Consider a two-photon excitation process where the wavenumber of the excitation light is 10000cm1. Assume an internal conversion of 6.891020J. If the fluorescence from both a single photon excitation (SPE) process and TPEF is 1000 photons/second, what is the intensity of the excitation light used in the TPEF process? Assume that 1=11016cm2,2=100GM, and I ISPE =5.32W/cm2. 1 photon s1cm2 3.651025 photon s1cm2 13.31050 photon s1cm2 1.331019 photon s1cm2
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