Consider present research aims to determine lycopene amount in homemade tomato paste. Within the scope of this study, firstly lycopene standard solutions were prepared with 20, 30, 40, 75 and 150 ppm concentrations and after HPLC analyses of these standards, the calibration curve would be drawn. While evaluating the results, the researchers decided that there was something wrong with the analyses and so it was necessary to repeat the experiment. Why do you think the researchers might have made such a decision? Also, where could they have made a mistake? Please draw calibration curve and explain using calibration curve.

Concentrations (ppm)	Peak Area (mAU*min)
20	1000
30	2500
40	4200
75	10500
150	14250