Consider the following reaction at 25C: Glucose + Pi Glucose-6-phosphate + H2O AG=+3 kcal/mol A) What is the value of Keg for this reaction? (Note: The concentration of water is ignored in these calculations.) B) If under physiologic (steady-state) conditions the concentration of glucose is 5 mM, the concentration of glucose-6-phosphate is 83 mM, and the concentration of Pi is 1 mM, will the reaction be allowed to proceed spontaneously from left to right? (Note: The concentration of water is ignored in these calculations.) C) If the reaction does not proceed spontaneously, what concentration of glucose would allow the reaction to proceed from left to right assuming the concentrations of the other reactants and products are the same as noted above? D) In the metabolic pathway known as glycolysis, the above reaction (Glucose + Pi Glucose-6- phosphate + HzO) is coupled to the following reaction: ATP + H20 ADP + Pi The steady-state concentrations of ATP and ADP in a generic human cell are 2.24 mM and 0.25 mM. respectively. Discuss why this coupling may be used in the cell.

Consider the following reaction at 25C Glucose + 1 + Glucose-6-phosphate + H0 AG" - +3 kcal/mol A) What is the value of K., for this reaction? (Note: The concentration of water is ignored in these calculations) B) If under physiologie (steady-state) conditions the concentration of glucose is 5 mm, the concentration of glucose-6-phosphate is 83 mm, and the concentration of Pi is 1 mM, will the reaction be allowed to proceed spontaneously from left to right? (Note: The concentration of water is ignored in these calculations.) C) If the reaction does not proceed spontaneously, what concentration of glucose would allow the reaction to proceed from left to right assuming the concentrations of the other reactants and products are the same as noted above? D) In the metabolic pathway known as glycolysis, the above reaction (Glucose + Pie Glucose-6- phosphate + H2O) is coupled to the following reaction: ATP+H30 ADP + Pi The steady-state concentrations of ATP and ADP in a generic human cell are 2.24 mM and 0.25 mm spectively Discuss why this coupling may be used in the cell