Consider the following single-molecule set up: Dye: N-(6-tetramethylrhodaminethiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3phosphoethanolamine, triethylammonium salt (TRITC DHPE; T-1391, Molecular Probes)

Excitation/emission: 540nm/566nm

Quantum yield: 0.5

Objective oil index of refraction: 1.5

Numerical aperture: 1.3

Excitation light: 514nm,57kW/cm2

Exposure time: 5ms

Transmittance Information Objective: 40%

Dichroic: 90%

Emitter: 99%

Tube lens: 90%

Camera detection efficiency: 40%

One-photon absorption cross section for rhodamine: =1016cm2

, the light bending angle for the objective

Based on the optics setup, what is the percentage of total fluorescence that reaches the camera?

Choose from the following options: 20% ; 32% ; 8%