Consider the following single-molecule set up: Dye: N-(6-tetramethylrhodaminethiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3phosphoethanolamine, triethylammonium salt (TRITC DHPE; T-1391, Molecular Probes) Excitation/emission: 540nm/566nm Quantum yield: 0.5 Objective oil index of refraction: 1.5 Numerical aperture: 1.3 Excitation light: 514nm,57kW/cm2 Exposure time: 5ms Transmittance Information Objective: 40% Dichroic: 90% Emitter: 99% Tube lens: 90% Camera detection efficiency: 40% One-photon absorption cross section for rhodamine: =1016cm2 , the light bending angle for the objective Estimate the fluorescence signal detected in photons per second. \begin{tabular}{c} 235,200 \\ \hline 7,840,000 \\ \hline 627,200 \\ \hline 470,400 \end{tabular}