-Convert absorbance to g of phenol using your standard phenol curve, divide by 10 to get g of phenol per minute, and plot g of phenol per minute against substrate concentration.

-Determine Km and Vmax (Lineweaver-Burk Equation)

Table 4. The effect of enzyme concentration on reaction rate No Buffer pH 10 Substrate, ml ml 1 1 1 2 1 1 3 1 1 4 1 1 5 1 1 6 1 1 | | Deionised water, Enzyme susp. Absorbance ml ml 1.0 0 0.000 (Blank) 0.8 0.2 0.036 0.6 0.4 0.059 0.4 0.6 0.090 0.8 0.176 0 1.0 0.320 0.2 Table 4. The effect of enzyme concentration on reaction rate No Buffer pH 10 Substrate, ml ml 1 1 1 2 1 1 3 1 1 4 1 1 5 1 1 6 1 1 | | Deionised water, Enzyme susp. Absorbance ml ml 1.0 0 0.000 (Blank) 0.8 0.2 0.036 0.6 0.4 0.059 0.4 0.6 0.090 0.8 0.176 0 1.0 0.320 0.2