Define(a) elution (e) retention time (i) longitudinal diffusion(b) mobile phase (f) retention factor ( j) eddy diffusion(c) stationary phase (g) selectivity factor (k) column resolution(d) distribution constant (h) plate height (l) eluent

Describe the general elution problem?

List the variables that lead to zone broadening in chromatography?

What are the major differences between gas-liquid and liquid-liquid chromatography?

What are the differences between liquid-liquid and liquid-solid chromatography?

What variables are likely to affect the selectivity factor a for a pair of analytes?

Describe how the retention factor for a solute can be manipulated?

Describe a method for determining the number of plates in a column?

Name two general methods for improving the resolution of two substances on a chromatographic column?

Why does the minimum in a plot of plate height versus flow rate occur at lower flow rates with LC than with GC?

What is gradient elution?

What would be the effect on a chromatographic peak of introducing the sample at too slow a rate?