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Describe how you would go about making up the following solutions (show all calculations). 1. 75ml of a 3M Kac (Potassium acetate), pH4.8 (3) 2.
Describe how you would go about making up the following solutions (show all calculations). 1. 75ml of a 3M Kac (Potassium acetate), pH4.8 (3) 2. 2.5L of a 1X TBE soln from an 8X stock solution (2) 3. 150ml GTE (glucose-tris-EDTA) containing, 35Mm Glucose, 25Mm Tris pH8, 15Mm EDTA. (6) 4. 145ml of a 10%(w/v) Glucose solution. (2) 5. 85ml of a 50mMMgCl solution, pH7.5. (3) 6. 150ml of a 75%(v/v) Ethanol solution. (2) Molecular weights Glucose =180.2g/mol Tris =121.14g/mol EDTA =372.2g/mol MgCl2=203.3g/molKaC=98.2g/molNaCl=58.44g/mol
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