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! ! ! Detailed Solution Needed The simple Michaelis - Menten model does not deal with all aspects of enzyme - catalyzed reactions. The model
Detailed Solution Needed The simple MichaelisMenten model does not deal with all aspects of enzymecatalyzed reactions. The model must be modified to treat the phenomena of inhibition. The inhibition process in general may be represented by the following sixstep scheme, in which I is the inhibitor, EI is a binary enzymeinhibitor complex, and EIS is a ternary enzymeinhibitorsubstrate complex. EIS EIS EIS In steps and S and I compete for sites on E to form the binary complexes ES and EI In steps and the ternary complex EIS is formed from the binary complexes. In steps and ES and EIS form the product ; if EIS is inactive, step is ignored. Treatment of the full sixstep kinetic scheme above with the PSSH leads to very cumbersome expressions for etc., such that it would be better to use a numerical solution. However, these can be greatly simplified if we assume the first four steps are at equilibrium, and With these assumptions, show that: where dissociation constant for and dissociation constant for EIS
Detailed Solution Needed
The simple MichaelisMenten model does not deal with all aspects of enzymecatalyzed reactions. The
model must be modified to treat the phenomena of inhibition.
The inhibition process in general may be represented by the following sixstep scheme, in which I is the
inhibitor, EI is a binary enzymeinhibitor complex, and EIS is a ternary enzymeinhibitorsubstrate
complex.
EIS
EIS
EIS
In steps and S and I compete for sites on E to form the binary complexes ES and EI In steps
and the ternary complex EIS is formed from the binary complexes. In steps and ES and EIS
form the product ; if EIS is inactive, step is ignored.
Treatment of the full sixstep kinetic scheme above with the PSSH leads to very cumbersome expressions
for
etc., such that it would be better to use a numerical solution. However, these can be greatly
simplified if we assume the first four steps are at equilibrium, and With these
assumptions, show that:
where dissociation constant for
and dissociation constant for EIS
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