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Determination of Ephedrine HCl in injection solution is carried out using the following procedure: - 1.0ml of the injection was diluted with distilled water until

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Determination of Ephedrine HCl in injection solution is carried out using the following procedure: - 1.0ml of the injection was diluted with distilled water until 100.0ml - 20.0ml of the resulting solution was further diluted with distilled water until 100.0ml - The sample was measured at 290nm, absorbance of 0.542 was observed - If the absorbance of 40.0ppm Ephedrine HCl is 0.548 Calculate the concentration of ephedrine HCl in the injection solution (in %) Solution of compound X which was measured using a cuvette (thickness 1cm ) had an absorbance of 0.500 at wavelength of 273nm. This solution was prepared by dissolving 50mg of compound X(BM200) in 250.0ml of hexane. QUESTION : b. What is the specific absorption (E1\%,1 cm ) of compound X ?)

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