1. Draw a schematic diagram of an optical system design for flow cytometry measurements of cells stained with antibodies conjugated with fluorescent dyes FITC and Texas Red:

1. Show how lasers can be spatially arranged for simultaneous excitation of 2 dyes
2. Incorporate FSC and SSC channels in the optical design
3. Indicate transmission characteristics of all optical filters and dichroic mirrors allowing simultaneous excitation of 2 dyes, separation of the scattered light and the fluorescent light emitted by 2 dyes