During a test of kinetics of an enzyme$-$catalyzed reaction, the following data were

recorded:

a$.$ Determine the $K_m$ and $V_{\text{m}\text{a}\text{x}}$ values for the reaction with no inhibitor present at

$30C($for both two enzyme concentrations$)$ and at $49\mathrm{.}6C.$ Interpret the effect

of enzyme concentration and temperature on $K_m$ and $V_{\text{m}\text{a}\text{x}}$ values.

Apply Eadie$-$Hofstee plot by using both millimetric paper and Excel

software. Calculate the constants by using both types of graphs and

compare.

b$.$ Determine the $K_i$ for the inhibitor at $30C$ and decide what type of inhibitor is

being used.

Apply Lineweaver$-$Burk plot by using both millimetric paper and Excel

software. Calculate the constants by using both types of graphs and

compare.