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During the lab you will prepare 100 ml of Tris buffer at pH=8.0 using the conjugate acid, trisHCl, and the conjugate base, Tris, (Tris has
During the lab you will prepare 100 ml of Tris buffer at pH=8.0 using the conjugate acid, trisHCl, and the conjugate base, Tris, (Tris has a pKa =8.1.).
a. Using the Henderson-Hasselbalch equation, determine the concentrations of both [A-] = tris = x and [HA] = trisHCl = (0.2 - x).
b. Calculate masses as needed.
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Organic Chemistry
Authors: Francis A. Carey
4th edition
0072905018, 978-0072905014
