Dynamic oxygen consumption rate: Use the data provided on excel spreadsheet

and $($a$)$ calculate oxygen consumption rate $($OCR$)$ in mM$/$min for a cell culture at

$24$ Million viable cells$/$ml$,$ and $($b$)$ calculate cell specific oxygen consumption rate

in umol$/$cell$/$hr$.$ Remember $100\%$ air saturation means $0\mathrm{.}2$ mM of oxygen in

solution.

$2.$ Using the data $($pH versus time$)$ provided in Excel spreadsheet, calculate mixing

time for the bioreactor at $10,000$L scale at different agitation rates. Plot the

mixing time against agitation rates $($rpm$).$ The bioreactor uses a pitched blade

impeller.

$3.$ Using the data $($DO versus time$)$ provided in Excel spreadsheet, calculate kl a for

oxygen at different agitation rates and at different gas flow rates. Make a plot of kla

against agitation rate and another plot showing kl a against gas flow rate. If you use Rate

method in addition to integral method, you will get extra point.

DATA

Kla Test, $2500$L bioreactor, pitch impeller blades

Condition: $2$LPM Flow, $300$ RPM

Time$($sec$)$ Time$($min$)$ DO$\%$

$00\mathrm{.}05$

$1542\mathrm{.}615$

$2995\mathrm{.}025$

$4577\mathrm{.}635$

$62810\mathrm{.}545$

$82513\mathrm{.}855$

$107317\mathrm{.}965$

$138423\mathrm{.}175$

$181330\mathrm{.}285$

$250441\mathrm{.}795$

Condition: $2$LPM Flow, $375$ RPM

Time$($sec$)$ Time$($min$)$ DO$\%$

$00\mathrm{.}05$

$951\mathrm{.}615$

$1913\mathrm{.}225$

$3085\mathrm{.}135$

$4377\mathrm{.}345$

$5889\mathrm{.}855$

$79613\mathrm{.}365$

$104317\mathrm{.}475$

$133122\mathrm{.}285$

$186031\mathrm{.}095$

Condition: $2$LPM$,450$ RPM

Time$($sec$)$ Time$($min$)$ DO$\%$

$00\mathrm{.}05$

$751\mathrm{.}315$

$1232\mathrm{.}125$

$1762\mathrm{.}935$

$2404\mathrm{.}045$

$3175\mathrm{.}355$

$4257\mathrm{.}165$

$5949\mathrm{.}975$

$93515\mathrm{.}685$

$199133\mathrm{.}295$

Condition: $1\mathrm{.}5$ LPM$,375$ RPM

Time$($sec$)$ Time$($min$)$ DO$\%$

$00\mathrm{.}05$

$1362\mathrm{.}315$

$2574\mathrm{.}325$

$3936\mathrm{.}535$

$5689\mathrm{.}545$

$77312\mathrm{.}955$

$105017\mathrm{.}565$

$143123\mathrm{.}875$

$207934\mathrm{.}785$

$356859\mathrm{.}595$

Condition: $0\mathrm{.}7$ LPM$,375$ RPM

Time$($sec$)$ Time$($min$)$ DO$\%$

$00\mathrm{.}05$

$1592\mathrm{.}615$

$3205\mathrm{.}325$

$5288\mathrm{.}835$

$77312\mathrm{.}945$

$106617\mathrm{.}855$

$146624\mathrm{.}465$

$199133\mathrm{.}275$

$260943\mathrm{.}585$

$340556\mathrm{.}795$