Enzymes called protein kinases catalyze the transfer of a phosphate group from a molecule of ATP onto a protein substrate molecule in a process called protein phosphorylation:

ATP + protein ADP + phosphorylated protein

The Bcr-Abl protein kinase is a mutated, hyperactive version of a protein kinase. Bcr-Abl is found in patients with chronic myelogenous leukemia (CML), a form of blood cancer. Bcr-Abl promotes cancer by rapidly catalyzing the phosphorylation of proteins that stimulate cell proliferation in their phosphorylated form. The Bcr-Abl enzyme displays Michaelis-Menten kinetics.

The parameters for this enzyme are:

• Km = 40 uM
• Vmax = 3 pmol/min

Calculate the initial reaction velocity (v) of Bcr-Abl-catalyzed reactions when protein substrate (s) is present at the following concentrations.

*Your answers must have units of pmol/min. Please type the units in with your answer*

• [ s ] = 0.0001 uM =
• [ s ] = 0.0003 uM =
• [ s ] = 0.0009 uM =
• [ s ] = 10 uM =
• [ s ] = 20 uM =
• [ s ] = 40 uM =
• [ s ] = 80 uM =
• [ s ] = 160 uM =
• [ s ] = 320 uM =
• [ s ] = 25,000 uM =
• [ s ] = 2,500,000 uM =
• [ s ] = 5,000,000 uM =