Example $3\mathrm{.}3$

Rates of hydrolysis of $p-$nitrophenyl$--$D$-$glucopyranoside by $-$glucosidase, an irreversible unimolecular reaction, were measured at several concentrations of the substrate. The initial reaction rates were obtained as given in Table $3\mathrm{.}2.$ Determine the kinetic parameters of this enzyme reaction.

Table $3\mathrm{.}2$ Hydrolysis rate of $p-$nitrophenyl$--$D$-$glucopyranoside by $-$glucosidase.

$\backslash$table$[[$Substrate concentration $(gmol{m}^{-3}),$Reaction rate $(gmol{m}^{-3}{s}^{-1})$