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Example 3 . 3 Rates of hydrolysis of p - nitrophenyl - - D - glucopyranoside by - glucosidase, an irreversible unimolecular reaction, were measured

Example 3.3
Rates of hydrolysis of p-nitrophenyl--D-glucopyranoside by -glucosidase, an irreversible unimolecular reaction, were measured at several concentrations of the substrate. The initial reaction rates were obtained as given in Table 3.2. Determine the kinetic parameters of this enzyme reaction.
Table 3.2 Hydrolysis rate of p-nitrophenyl--D-glucopyranoside by -glucosidase.
\table[[Substrate concentration (gmolm-3),Reaction rate (gmolm-3s-1). Explain it in detail.
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