Exercise 15 Alanine Aminotransferase Determination

Put your own values that satisfies the question

1. Observation and Evaluation of Results

• Compute for the values of Cn, Cp and U (normal)

• Evaluate your results by answering the following questions:

  • Is the test valid? Explain your answer. Can you release the result?

  • Does the patient have the disease? Explain.

Review Questions

1. Give the substrate used in the assay. Identify the donor and recipient molecule.

2. To what condition is ALT most indicated?

3. Give the underlying principle of the Reitman-Frankel method. Identify the following:

   1. End product

   2. Color reagent to measure the end product

   3. Co-factor used.