Experiment 2: Effect of Temperature on Enzyme Activity

Result Tables

Table 2: Balloon Circumference vs.Temperature

Tube	Temperature (°C)	Balloon Circumference (Uninflated; cm)	Balloon Circumference (1 minute; cm)	Balloon Circumference (3 minutes; cm)
1 – Room Temperature
2 – Hot
3 – Cold

Post-Lab Questions

1. What reaction is being catalyzed in thisexperiment?
2. What is the enzyme in this experiment? What is thesubstrate?
3. What is the independent variable in this experiment? What isthe dependent variable?
4. How does the temperature affect enzyme function? Use evidencefrom your data to support your answer.
5. Use Excel or another graphing program to create a graph of thechange in balloon circumference over time for each differenttemperature. Insert an image of your graph here.
6. Why is measuring the changes in balloon circumference aneffective way to measure the activity of this specific enzyme?
7. Is there a negative control in this experiment? If yes,identify the control. If no, suggest how you could revise theexperiment to include a negative control.
8. In general, how would an increase in substrate alter enzymeactivity?
9. Design an experiment to determine the optimal temperature forenzyme function, complete with controls. Where would you find theenzymes for this experiment? What substrate would you use?