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Figure 1 (overleaf) shows a chromatogram of a lab scale separation of proteins and weakly retained impurities, peaks 2 to 4 being the retained Proteins.

Figure 1 (overleaf) shows a chromatogram of a lab scale separation of proteins and weakly retained impurities, peaks 2 to 4 being the retained Proteins. The void peak time for the separation is given as t0 = 0.83 mins. (i) Determine the retention factors for each peak (ii) If the column is 30 cm long, what is the theoretical plate height (in m) for each peak?

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