Fluorescence titration Prepare a fluorescence cuvette containing 2 mL of a CT DNA and acridine orange solution that is 8.5 uM in CT DNA and 2.5 um in acridine orange (make a plan and discuss it with your instructor). Incubate this solution for 5 minutes, and perform the first fluorescence scan from 510 to 700 nm. This is the fluorescence of DNA-AO Add 3.4 ul of resveratrol from stock solution 5 mM (each addition increases the concentration of resveratrol in solution by 1 equivalent - 8.5 uM) and wait for 1 minute. Measure fluorescence. Repeat for a total of 20 additions. Emission of DNA-AO is monitored at 525 nm. Save all your data as .txt files. UV-visible titration Prepare a V-visible cuvette containing 2 mL of a resveratrol solution 25 M (make a plan and discuss it with your instructor). Perform the first scan from 200 nm to 500 nm. Add 10 uL of CT DNA solution from stock 340 um concentration (each addition will increase the DNA concentration by 1.7 uM), incubate for 1 minute and scan again. Repeat for a total of 15 additions. The absorbance of resveratrol is monitored at 306 nm. 2. Perform the UV-visible and fluorescence titrations as per the instructions in the manual UV-visible cuvette Fluorescence cuvette 10 ul additions CT DNA (340 uM) 3.4 ul additions Resveratrol 25 8.5 UM DNA 2.5 ? ul buffer ? ul resveratrol stock solution Resveratrol ? ul buffer ? ul DNA stock solution (340 uM) ? uL AO stock solution 2 mL 2 mL Fluorescence titration Prepare a fluorescence cuvette containing 2 mL of a CT DNA and acridine orange solution that is 8.5 uM in CT DNA and 2.5 um in acridine orange (make a plan and discuss it with your instructor). Incubate this solution for 5 minutes, and perform the first fluorescence scan from 510 to 700 nm. This is the fluorescence of DNA-AO Add 3.4 ul of resveratrol from stock solution 5 mM (each addition increases the concentration of resveratrol in solution by 1 equivalent - 8.5 uM) and wait for 1 minute. Measure fluorescence. Repeat for a total of 20 additions. Emission of DNA-AO is monitored at 525 nm. Save all your data as .txt files. UV-visible titration Prepare a V-visible cuvette containing 2 mL of a resveratrol solution 25 M (make a plan and discuss it with your instructor). Perform the first scan from 200 nm to 500 nm. Add 10 uL of CT DNA solution from stock 340 um concentration (each addition will increase the DNA concentration by 1.7 uM), incubate for 1 minute and scan again. Repeat for a total of 15 additions. The absorbance of resveratrol is monitored at 306 nm. 2. Perform the UV-visible and fluorescence titrations as per the instructions in the manual UV-visible cuvette Fluorescence cuvette 10 ul additions CT DNA (340 uM) 3.4 ul additions Resveratrol 25 8.5 UM DNA 2.5 ? ul buffer ? ul resveratrol stock solution Resveratrol ? ul buffer ? ul DNA stock solution (340 uM) ? uL AO stock solution 2 mL 2 mL
