For immunohistochemistry, sections were washed three times in PBS containing 0.25% Tween 20 and then stained following the manufacturer's instructions for Vectastain ABC kit, Elite PK-6100 standard ImmPact tm DAB peroxidase Substrate kit SK-4105 (Vector Lab). The sections were then mounted on slides and dried overnight at 55C. After soaking in Xylene two times for 2 min each, the slides were sealed with Permount (Vector Lab)