Genus identification following the flow chart: ENTEROTUBE OBSERVATIONS To highlight an observation on the observation column, either change the color of the word or bold the word. For example on the first row there are two options Red or Yellow so if the observation is yellow then make the word Yellow like this Yellow or like this Yellow. Remember that only the positive results are counted towards the 5-digit number. Use the image on the next page to guide you to get the 5-digit number that you will look for in the enterotube code book. You can also highlight the number if it is a positive result. Following the example above with the Yellow word then also highlight the number 2 since Yellow is a positive result Enterotube Compartment 1 GLU-GAS 1 GLU-GAS 2 LYS 3 ORN 4 HS/IND TWO tests in this compartment SADON 6 LAC 7 ARAB 8 SORB loun Shigella Media Glucose fermentation Gas from glucose fermentation Lysine decarboxylase Omithine decarboxylase a) HS production b) Indole production Note: last test after Kovac's reagent added Adonitol fermentation Lactose fermentation Arabinose fermentation Sorbitol fermentation Observation (change color of word or bold word) Positive Negative Red Wax not separated Yellow Yellow a) no change b) no change Red Red Red Red Yellow Wax Separated Purple Purple a) Black Number 2 Yellow Yellow Yellow Yellow 1 4 2 1 b)Red after Kovac's 4 2 2 10 DUL-PA TWO tests in this compartment 11 UREA 12 CIT Voges Proskauer a) Dulcitol b) Phenylalanine deamination Urease Citrate utilization Styles Green b) Not smoky Peachy Green a) Yellow b)Smoky Bright pink Blue Select- Editing 2 1 L 1. Was the genus given by the flow chart the same as the genus given by the enterotube? Explain why? No. They were different. When more testing is done you can figure out the correct species. 2. Should the same bacterium have been identified? Explain. Remember that genus is not the same thing as species. THINK critically about presumptive identification vs confirmed identification. 3. What is enteric bacteria? Why are they medically and clinically important? 4. Differentiate a coliform, an enteric that is not a coliform, and enterococci. Give an example of an organism in each group 5. Is it possible for a bacterium to ferment lactose but not glucose? Look at the composition of the sugars and explain your answer.