Given below is is the experimental data performed when the substrate Laughing is hydrolyzed by 5 nM of the enzyme Hola, with and without the presence of the inhibitor Love. The reaction volume is 12 uL for each.

Laughing V (+3 nMLove) umol/sec V (+10 nMLove)umol/sec V ( + 0 nMLove)umol/sec

2 12 11 13 3 17 17 19 5 30 24 38 10 39 31 58 20 55 42 70 40 62 40 81

1. Plot the Michaelis Menten and Lineweaver burke curve with all 3 conditions. 2. What is the Km and Vmax by estimation using the Michaelis Menten plot? 3. Calculate the km and vmax using the line weaver burke plot.

4. What difference(s) is in the value for Km from b and c and why? 5. What type of inhibitor would this be?