Question
Glucose is converted into glutamic acid in many mammalian cells through the following reaction: C 6 H 12 O 6 + NH 3 + O
Glucose is converted into glutamic acid in many mammalian cells through the following reaction:
C6H12O6 + NH3 + O2 C5H9NO4 + CO2 + H2O
Additionally, mammalian cells can be placed in bioreactors and the biochemical conditions optimized to convert glucose to glutamic acid. Assume a simple bioreactor system that contains mammalian cells. The inlet flow rate of C6H12O6 is 1.50 102 mol/day. NH3 is delivered at a rate of 1.70 102 mol/day. O2 is provided such that 1.60 102 mol/day are dissolved in liquid (i.e., accessible to the cells for uptake). Assume that the reaction goes to completion.
a) Balance the reaction, given that RQ = 0.43. Determine the limiting reagent, the reaction rate, R, and the fractional conversions of C6H12O6, NH3, and O2.
b) Calculate the molar and mass flow rates of all constituents leaving the bioreactor including the products and excess reactants.
c) Confirm that total mass is conserved and that total moles is not.
HINT 1: RQ = Respiratory Quotient HINT 2: MATLAB may be helpful for solving a set of alg ebraic equations.
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