Glycolysis 1. On the attached picture of glycolysis label all of the intermediates and enzymes. 2. On the attached picture circle the primary regulation point for glycolysis. Next to the step list the inhibitors for this step in red and the activators in green. 3. Label carbon one of glucose by coloring it blue. Follow this carbon through glycolysis by shading the appropriate carbon blue in all of the subsequent intermediates. If a molecule of glucose that was radiolabelled at C-1 went through glycolysis, where would the radioactive label be in the resulting pyruvate? Draw pyruvate and circle this carbon. 4. Repeat your labelling experiment with carbon number 6. Use a new color. Where is the label in the resulting pyruvate? Draw pyruvate and circle the carbon. 5. Where would the label be in the resulting pyruvate if fructose labelled at C-1 underwent glycolysis? Draw pyruvate and circle the carbon. OH OH . HC O 2 3 4 3 6 OH CHOH co CHOH + HCO HOOH ATP ADP P+HO 0 6 CHO HOOH OH HOOH HOOH 00 ADP ATP PO H. O OH 000 OH CHOH HOCH CHOH OH CH,O 2X NAD. 000 DOOH = HOOH HOH NADH + H ATP o'll ADP 000 CH POCH, A ADP OH 10 HOOH ATP HOOH CHOP OH CH,O poor , coo ( CH