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group should run one sample consisting of 4 ug of a. DNA digested with HindlII, the proper volume of loading dye and TE buffer,
group should run one sample consisting of 4 ug of a. DNA digested with HindlII, the proper volume of loading dye and TE buffer, in a total volume of 15 ul. Knowing that the stock concentration of the DNA digested with Hindlll is 432 ug/ml, what volume (in microliters) of DNA, TE buffer, and 6x loading dye would be mixed together to get a total volume of 15 ul? Each LAMBDA (A) 40,000 48,502 10,000 20,000 30,000 EcoRI sites 44,972 31,747 21,226 26,104 39,168 *-5,804*3,530 *4,878*s.643-* 7,421 -21,226- 37,459 HindlII sites 36.895 37.584 44,141 23,130 25,15727.479 **-6.557*4,361-> 564 125 9.416 23.130- 2.0272.322 Restriction Digest Map of Figure 3.1 lambda DNA
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