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help me please The following data on bakers' yeast in a particular medium at 23.4C and various oxygen partial pressures were obtained: where PO2 is
help me please
The following data on bakers' yeast in a particular medium at 23.4C and various oxygen partial pressures were obtained: where PO2 is the partial pressure of oxygen (in mmHg ) and QO2 is the rate for oxygen uptake (in l of O2 per hour per mg of cells). You can consider that PO2 and Qo2 represent substrate concentration and rate of enzymatic reaction, respectively. Lineweaver-Burk transformation was employed to obtain MichaelisMenten equation in the linear form. The Lineweaver-Burk plot is displayed below. The solid line represents the case without sulfanilamide, an inhibitor), while the dashed line represents the case with 20mg/ml of sulfanilamide. The linear regression models obtained from Microsoft Excel are located next to the corresponding straight lines in the plot. Answer the following questions. a) What are the values of the maximum Q02(Vmax) and the Michaelis constant (KM) when no sulfanilamide was added? b) What is the type of enzyme inhibition caused by addition of sulfanilamide? Explain your reasoning. c) What is the value of K1 for this type of enzyme inhibition? d) If we reduced the amount of sulfanilamide added to the medium from 20 to 10mg/ml, what is the rate for oxygen uptake when the partial pressure for oxygen is 2.0mmHg Step by Step Solution
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