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help Which factors could explain no emission at the expected FRET wavelength? The emission and excitation wavelengths of the fluorescent proteins are overlapping. The fusion

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Which factors could explain no emission at the expected FRET wavelength? The emission and excitation wavelengths of the fluorescent proteins are overlapping. The fusion proteins are inactive or unstable. The donor and the acceptor are too close for resonance energy transfer between them. The hybrid proteins, with bulky fluorophores attached, do not interact normally

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