hey guys!

can anyone tell me what i should put for the corrected absorbance at 500nm ?? is that discovered when i do a "graph" or is it the same as absorbance at 500nm?

"Graph 1.2: Plot the standard curve for this experiment. The x-axis should represent the concentration of the standard samples (units mol/mL). Use Excel or Google sheets to plot your graph. "

Ensure that you;

• include an appropriate title, label the axes (identity and units), graph key, logical scale, line of best fit included, graph should be neat and easy to read
• Provide an appropriate figure legend describing what the graph shows (figure title/scope, description of the standard data presented (must include units), method; how data were obtained, trendline description

Thanks

SAMPLE NO #459 Table 1.3: Standard Curve (10 marks) Tube Number 1 2 3 4 5 6 0 0.2 0.4 0.6 0.8 1.0 Final concentration of glucose (umol/mL) Total volume in tube (mL) 0.2 0.2 0.2 0.2 0.2 0.2 | 0 0.04 0.08 0.12 0.16 0.2 Volume of 1.0 mM glucose (mL) Volume of water (mL) 0.2 0.16 0.12 0.08 0.04 0 Absorbance at 500 nm 0.100 0.132 0.254 0.362 0.467 0.592 Corrected Absorbance at 500nm Table 1.4: Unknown Sample Tube Number 7 8 9 10 11 12 13 14 15 16 17 18 Undiluted Unknown solution (ML) - - ! 1/3 dilution of Unknown solution (mL) 0.2 0.2 0.2 . - 1/9 dilution of Unknown solution (mL) - 0.2 0.2 0.2 . ! 1/27 dilution of Unknown solution (mL) 0.2 0.2 0.2 Absorbance at 500nm 0.522 0.571 0.502 0.192 0.178 0.185 0.075 0.066 0.069 - - - - - - - - - - - Corrected Absorbance at 500nm