Hi! I need some help understanding a qPCR dataset.

So I tested a KO and WT group for Nampt gene expression. My supervisor told me that we would look at the "relative" values. So she made a standard curve from all samples by mixing equal amounts of each and then she diluted it.

She then took log to the amount and I am not sure why? What does log do in this context?

The CT values was used to get a mean ct, and the mean Ct and log amount was used to plot a standard curve. I am just not sure why log was needed.

We used the slope and y-axis to calculate the relative amount. The amount was a log amount, and we then used the command "potent" in excel. To delete log I guess? I am not sure how log works.

Hope somebody can help me!

Thanks a lot!

Ct 1 Ct 2 Ct 3 Mean SD AMOUNT log (AMOUNT) 1,0000 0,0000 0,5000 -0,3010 0,2500 -0,6021 0,1000 -1,0000 0,0500 -1,3010 0,0100 -2,0000 0,0000 29,24 30,05 31,02 32,48 33,35 33,54 29,13 30,42 31,23 32 29,04 30,28 30,74 32,29 33,16 33,29 29,14 30,25 31,13 32,15 33,26 33,42 0,100 0,187 0,148 0,205 0,134 0,177 33,65 35,28 -3,0610 Slope: y-axis: 29,6190 R?= 0,9079 Ct 1 Ct 2 Ct 3 MEAN SD test Log amount AMOUNT 29,53 29,68 29,86 29,94 29,25 29,94 29,96 29,90 29,86 0,06 0,16 -0,09 -0,08 0,809 0,834 29,25 29,1 29,20 0,09 0,14 1,371 30 29,51 29,54 29,53 0,03 1,073 0,02 0,14 29,92 30,17 30,01 -0,13 29,93 29,51 0,747 1,069 29,55 30,01 29,53 0,03 0,03 Ct 1 Ct 2 Ct 3 Mean SD AMOUNT log (AMOUNT) 1,0000 0,0000 0,5000 -0,3010 0,2500 -0,6021 0,1000 -1,0000 0,0500 -1,3010 0,0100 -2,0000 0,0000 29,24 30,05 31,02 32,48 33,35 33,54 29,13 30,42 31,23 32 29,04 30,28 30,74 32,29 33,16 33,29 29,14 30,25 31,13 32,15 33,26 33,42 0,100 0,187 0,148 0,205 0,134 0,177 33,65 35,28 -3,0610 Slope: y-axis: 29,6190 R?= 0,9079 Ct 1 Ct 2 Ct 3 MEAN SD test Log amount AMOUNT 29,53 29,68 29,86 29,94 29,25 29,94 29,96 29,90 29,86 0,06 0,16 -0,09 -0,08 0,809 0,834 29,25 29,1 29,20 0,09 0,14 1,371 30 29,51 29,54 29,53 0,03 1,073 0,02 0,14 29,92 30,17 30,01 -0,13 29,93 29,51 0,747 1,069 29,55 30,01 29,53 0,03 0,03