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HI , I REALLY NEED THESE QUESTIONS ANSWERED AS SOON AS POSSIBLE PLEASE! Thank you! GLP - 1 receptor agonists like Semaglutide ( Ozempic ,

HI, I REALLY NEED THESE QUESTIONS ANSWERED AS SOON AS POSSIBLE PLEASE! Thank you!
GLP-1 receptor agonists like Semaglutide (Ozempic, Wegovy) are a group of compounds that help stabilize blood sugar in Type II Diabetics. A small company wants to make approximately 8000 doses of Semaglutide by making the precursor protein for Semaglutide via fermentation using the bacteria E. coli, with glucose as substrate and ammonium chloride as the source of nitrogen. Air is bubbled into the reactor to provide oxygen. At the start, 10 g/L of glucose and 7 g/L of ammonium chloride is added to 200 L of media. After 26 hours of batch operation, 0.2% by volume of glucose remains and a feed stream is started at a flow rate of 32.5 L/hr for 20 hours. The feed contains 600 g/L glucose and a certain concentration of ammonium chloride. At the end of the fed-batch, the reactor is harvested with 180 g/L cells, 10.8 grams of Semaglutide, and 5.73% by volume glucose. All of the ammonium chloride was consumed. The fermentation temperature is 37\deg C and the stirrer power is 5 kW. Assume no Semaglutide was present initially, and that starting cell concentration and trace nutrients were negligible.
Questions may not be in sequential order.
1) Calculate the yield of cells produced per gram glucose consumed, Y_XS, in g cells/g glucose.
2) How many kilograms of glucose were consumed over the entire fermentation?
3) Determine all the coefficients (a, b, c, d, e and f) for the general stoichiometric equation for E. coli cells producing Semaglutide from glucose by solving using simultaneuos equations.
4) To improve the sustainability of this process, the carbon dioxide generated is to be used in a biofuel production process. If 1.8% of the carbon dioxide generated over the course of the fermentation remains dissolved in the media, what mass of carbon dioxide can be supplied to the biofuel process? (Units in kg)
5) Calculate the flowrate of air bubbled into the bioreactor over the course of the fermentation given that air has a weight fraction of 23.3% oxygen and air is supplied at a 30% excess of the stoichiometric requirements. (Units in L/min)
6) Find the value of the yield coefficient, Y_PS, in g Semaglutide/g Glucose.
7) Assuming that the carbon dioxide produced remains the same, and the glucose consumption also remains the same, how much Semaglutide could we theoretically make if the system was operated at thermodynamic maximum? (Units in kg)
8) Determine the concentration of ammonium chloride in g/L that was present in the fed-batch feed stream.
9) Calculate the production rate of Semaglutide in mg/hr.
10) Estimate the heat duty, Q, for this fermentation in kJ given that 1.5% of the final reactor volume evaporated. Does this reactor need to be heated or cooled? Assume that \rho _media=\rho _water. (Answer should be in scientific notation)
POSSIBLY USEFUL INFO
General Stoichiometric Equation Including Product:
C_w H_x O_y N_z+aO_2+bH_g O_h N_i->cC_n O_o N_p+dCO_2+eH_2 O+fC_j H_k O_l N_m
Escherichia Coli CH_1.77 O_0.49 N_0.24 with 10% ash
Semaglutide C_11 H_12 N_2 O_2 Molecular Weight =4109
Glucose C_6 H_12 O_6 Molecular Weight =180
Ammonium chloride NH_4 Cl Molecular Weight =53.5
Oxygen O_2 Molecular Weight =32
Carbon dioxide CO_2 Molecular Weight =44
Densities: Glucose 1560 g /L, Air 1.135 g/L, Oxygen 1.331 g/L, Water 1.00 g/mL, Ammonium chloride 1.53 g/mL
Overall energy balance for bioreactor: -H_rxn-M_v h_v-Q+W_s=0
h_v of water at 37\deg C =2413.1 kJ/kg
H_rxn for aerobic reactions =460 kJ/mol O_2
Atomic masses: C=12, H=1, N=14, O=16, Cl=35.5
Conversions: 1g =1000 mg,1m3=1000 L,1L =1000 mL

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