HI$,$ I REALLY NEED THESE QUESTIONS ANSWERED AS SOON AS POSSIBLE PLEASE! Thank you!

GLP$-1$ receptor agonists like Semaglutide $($Ozempic$,$ Wegovy$)$ are a group of compounds that help stabilize blood sugar in Type II Diabetics. A small company wants to make approximately $8000$ doses of Semaglutide by making the precursor protein for Semaglutide via fermentation using the bacteria E$.$ coli, with glucose as substrate and ammonium chloride as the source of nitrogen. Air is bubbled into the reactor to provide oxygen. At the start, $10$ g$/$L of glucose and $7$ g$/$L of ammonium chloride is added to $200$ L of media. After $26$ hours of batch operation, $0\mathrm{.}2\%$ by volume of glucose remains and a feed stream is started at a flow rate of $32\mathrm{.}5$ L$/$hr for $20$ hours. The feed contains $600$ g$/$L glucose and a certain concentration of ammonium chloride. At the end of the fed$-$batch, the reactor is harvested with $180$ g$/$L cells, $10\mathrm{.}8$ grams of Semaglutide, and $5\mathrm{.}73\%$ by volume glucose. All of the ammonium chloride was consumed. The fermentation temperature is $37\backslash$deg C and the stirrer power is $5$ kW$.$ Assume no Semaglutide was present initially, and that starting cell concentration and trace nutrients were negligible.

Questions may not be in sequential order.

$1)$ Calculate the yield of cells produced per gram glucose consumed, Y$\_$XS$,$ in g cells$/$g glucose.

$2)$ How many kilograms of glucose were consumed over the entire fermentation?

$3)$ Determine all the coefficients $($a$,$ b$,$ c$,$ d$,$ e and f$)$ for the general stoichiometric equation for E$.$ coli cells producing Semaglutide from glucose by solving using simultaneuos equations.

$4)$ To improve the sustainability of this process, the carbon dioxide generated is to be used in a biofuel production process. If $1\mathrm{.}8\%$ of the carbon dioxide generated over the course of the fermentation remains dissolved in the media, what mass of carbon dioxide can be supplied to the biofuel process? $($Units in kg$)$

$5)$ Calculate the flowrate of air bubbled into the bioreactor over the course of the fermentation given that air has a weight fraction of $23\mathrm{.}3\%$ oxygen and air is supplied at a $30\%$ excess of the stoichiometric requirements. $($Units in L$/$min$)$

$6)$ Find the value of the yield coefficient, Y$\_$PS$,$ in g Semaglutide$/$g Glucose.

$7)$ Assuming that the carbon dioxide produced remains the same, and the glucose consumption also remains the same, how much Semaglutide could we theoretically make if the system was operated at thermodynamic maximum? $($Units in kg$)$

$8)$ Determine the concentration of ammonium chloride in g$/$L that was present in the fed$-$batch feed stream.

$9)$ Calculate the production rate of Semaglutide in mg$/$hr$.$

$10)$ Estimate the heat duty, Q$,$ for this fermentation in kJ given that $1\mathrm{.}5\%$ of the final reactor volume evaporated. Does this reactor need to be heated or cooled? Assume that $\backslash$rho $\_$media$=\backslash$rho $\_$water. $($Answer should be in scientific notation$)$

POSSIBLY USEFUL INFO

General Stoichiometric Equation Including Product:

C$\_$w H$\_$x O$\_$y N$\_$z$+$aO$\_2+$bH$\_$g O$\_$h N$\_$i$->$cC$\_$n O$\_$o N$\_$p$+$dCO$\_2+$eH$\_2$ O$+$fC$\_$j H$\_$k O$\_$l N$\_$m

Escherichia Coli CH$\_1\mathrm{.}77$ O$\_0\mathrm{.}49$ N$\_0\mathrm{.}24$ with $10\%$ ash

Semaglutide C$\_11$ H$\_12$ N$\_2$ O$\_2$ Molecular Weight $=4109$

Glucose C$\_6$ H$\_12$ O$\_6$ Molecular Weight $=180$

Ammonium chloride NH$\_4$ Cl Molecular Weight $=53\mathrm{.}5$

Oxygen O$\_2$ Molecular Weight $=32$

Carbon dioxide CO$\_2$ Molecular Weight $=44$

Densities: Glucose $1560$ g $/$L$,$ Air $1\mathrm{.}135$ g$/$L$,$ Oxygen $1\mathrm{.}331$ g$/$L$,$ Water $1\mathrm{.}00$ g$/$mL$,$ Ammonium chloride $1\mathrm{.}53$ g$/$mL

Overall energy balance for bioreactor: $-$H$\_$rxn$-$M$\_$v h$\_$v$-$Q$+$W$\_$s$=0$

$$h$\_$v of water at $37\backslash$deg C $=2413\mathrm{.}1$ kJ$/$kg

$$H$\_$rxn for aerobic reactions $=460$ kJ$/$mol O$\_2$

Atomic masses: C$=12,$ H$=1,$ N$=14,$ O$=16,$ Cl$=35\mathrm{.}5$

Conversions: $1$g $=1000$ mg$,1$m$3=1000$ L$,1$L $=1000$ mL