. High energy pulsed UV light source emits light energy of 90 mecm2 per pulse. Pulse frequency has been kept at 5 pulses per second for 30 s treatment. [absorption coefcient, I1 = 0.1 cm'l] (2.5 marks) a. Calculate the light energy ux at 3, T and 10 cm from lamp using BeerLambert's law after St) S. Plot the curve of energy per pulse (mecmg) vs distance. Also calculate the Dyalue of a microorganism at 1C! cm from lamp, if the Dvalues of the microorganism are 10 s and 85 s at 4 and 9 cm from lamp. Firstly, you need to calculate the energy per pulse at these distances and the 2 value