How many active sites are present in the DNA polymerase to catalyze the addition of the four dNTPs?

a) 1

b) 2

c) 3

d) 4

2. How does the polymerase recognize the correct dNTP for an addition?

a) Structure of dNTP

b) Molecular weight of dNTP

c) Purine and pyrimidine orientation

d) Ability of hydrogen bond formation

3. DNA polymerase can distinguish between dNTPs and rNTPS due to ___________

a) Structural differentiation

b) Steric exclusion

c) Steric hindrance

d) Enzyme substrate mismatch

4. When we compare the structure of DNA polymerase to the structure of a body part, it resembles to _________________

a) Right hand

b) Left hand

c) Right foot

d) Left foot

5. With respect to the palm domain of the DNA polymerase which of the following is not its property?

a) Contains primary elements of the catalytic site

b) Binds to 2 divalent ions

c) Composed of helix

d) Brings about the environmental changes around 3'-OH of dNTP

6. The two divalent metal ions of the active sites of DNA polymerase are major catalytic elements that bring about changes required for the joining of the dNTPs.

a) True

b) False

7. Mismatched DNA does not affect the rate of activity of DNA polymerase.

a) True

b) False

8. Which is the rate limiting step of DNA replication?

a) Formation of the RNA primer

b) Binding of primer to the DNA template

c) Binding of DNA polymerase to the primer:template junction

d) Binding of the first dNTP to the primer