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Bakers' yeast is produced in a 50,000 litre fermenter under aerobic conditions. The carbon substrate is sucrose; ammonia is provided as the nitrogen source.

 

Bakers' yeast is produced in a 50,000 litre fermenter under aerobic conditions. The carbon substrate is sucrose; ammonia is provided as the nitrogen source. The average biomass composition is CH183 O0.5s No.17 with 5% ash. Under conditions supporting efficient growth, biomass is the only major product and the biomass yield from sucrose is 0.5 g/g. If the specific growth rate is 0.45 h', estimate: a) oxygen requirement for each gram of biomass b) rate of oxygen consumption when the yeast concentration is 10 g/l. 4 c) the rate of heat removal required to maintain constant temperature in the fermenter when the yeast concentration is 10 g/l. The stoichiometric equation for this reaction: C12H22O11 + aO2 + 6NH3 > CCH1.83O0.55 No.17 + dCO2 + EH2O Hint: MW of sucrose = 342.3; biomass formula weight = 25.04; MW of oxygen 32, AHrxn for aerobic metabolism = - 460 kJ/gmol O2 consumed

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