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I am not sure about how to solve these questions. Some help will be appreciated :) 5. A particular enzyme acts by forming a hydrogen
I am not sure about how to solve these questions. Some help will be appreciated :)
5. A particular enzyme acts by forming a hydrogen bond with the transition state, a bond that is completely absent with either reactant or product. If the hydrogen bond is associated with 20kJ/mol, calculate how much the reaction would be accelerated at 25 degrees Celsius. 3204faster 6. A protein is mixed with ligand. After binding, the remaining concentration of free ligand is 2M. If the stoichiometry of the interaction is 2 ligands/protein, and the Kd is 100nM, then what is the fraction of enzyme that is not bound? 0.23% 7. Given the Kd for a protein with n equivalent, non-interacting sites, what is the fraction of proteins that have one or more ligands bound as a function of the free ligand concentration upon equilibrium ([L]) ? 1(1K1+[L][L])nStep by Step Solution
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