i. Ispeinesib inhibits ATP hydrolysis by Kinesin.

How would this affects the affinity of the protein for tubulin?

Explanation of the effect of drug is linked to effect of nucleotide state on affinity of motor to tubulin is required.

ii. Ispeinesib is being developed as a chemotherapeutic drug.

Explain the cellular process targeted by kinesin inhibition and why it would decrease cell division or metastasis in cancer cells.

Pick a part of cell division that might be affected by inhibition of kinesin.

Explain why inhibition of kinesin will inhibit this process.

minus end actin filament plus end Question 3-2. Mechanisms of action of chemotherapeutic drugs (16pts) a. Vinblastine is a chemotherapeutic drug that destabilizes microtubules. "The vinca alkaloids are dimeric compounds.... They act by binding specifically to B-tubulin and block its ability to polymerize with a-tubulin into microtubules. myosin head ATP i.Given your understanding of microtubule dynamics, rewrite this statement in terms that other students in the class will understand! Rubric (4): paraphrase is correct and unique. ATP myosin thick filament ATP HYDROLYSIS ii.Explain how this drug will prevent a cell from EXITING mitosis. Rubric (4): Explain why the change in MT dynamics caused by this drug will inhibit a cell from moving from M phase to interphase ADPP b. The figure at right outlines how myosin binds and hydrolyzes ATP to walk on a microfilament. The mechanism is very similar to how KINESIN walks so we will use this figure to answer the questions below! Ispinesib is a drug that specifically inhibits a kinesin motor protein called "Eg5". ADP i. ADP plus end i. Ispeinesib inhibits ATP hydrolysis by Kinesin. How would this affect the affinity of the protein for POWER STROKE tubulin? Rubric (4pts): Explanation of the effect of drug is linked to minus end effect of nucleotide state on affinity of motor to tubulin. Ispeinesib is being developed as a chemotherapeutic drug. Explain the cellular process targeted by kinesin inhibition and why it would decrease cell division or metastasis in cancer cells. Rubric (4): Pick a part of cell division that might be affected by inhibition of kinesin. Explain why inhibition of kinesin will inhibit this process. minus end actin filament plus end Question 3-2. Mechanisms of action of chemotherapeutic drugs (16pts) a. Vinblastine is a chemotherapeutic drug that destabilizes microtubules. "The vinca alkaloids are dimeric compounds.... They act by binding specifically to B-tubulin and block its ability to polymerize with a-tubulin into microtubules. myosin head ATP i.Given your understanding of microtubule dynamics, rewrite this statement in terms that other students in the class will understand! Rubric (4): paraphrase is correct and unique. ATP myosin thick filament ATP HYDROLYSIS ii.Explain how this drug will prevent a cell from EXITING mitosis. Rubric (4): Explain why the change in MT dynamics caused by this drug will inhibit a cell from moving from M phase to interphase ADPP b. The figure at right outlines how myosin binds and hydrolyzes ATP to walk on a microfilament. The mechanism is very similar to how KINESIN walks so we will use this figure to answer the questions below! Ispinesib is a drug that specifically inhibits a kinesin motor protein called "Eg5". ADP i. ADP plus end i. Ispeinesib inhibits ATP hydrolysis by Kinesin. How would this affect the affinity of the protein for POWER STROKE tubulin? Rubric (4pts): Explanation of the effect of drug is linked to minus end effect of nucleotide state on affinity of motor to tubulin. Ispeinesib is being developed as a chemotherapeutic drug. Explain the cellular process targeted by kinesin inhibition and why it would decrease cell division or metastasis in cancer cells. Rubric (4): Pick a part of cell division that might be affected by inhibition of kinesin. Explain why inhibition of kinesin will inhibit this process