I just want answers to table 4. Im not sure what to do Please can you explain your answers? Thank you very much!
Using the stock solution given, enter the volumes used to prepare the six standard solutions of albumin in NaCl solution to represent 40-fold dilutions of typical "patient" concentrations from 20 to 70 g/L. For each standard the target "patient" concentration is given, you need to record the actual concentration of the standard, the volume of the stock solution used to make the standard, and the volume of NaCl solution used to make the standard. We are assuming you are making 10 mL of each standard solution. NOTE: If you ever need to write a number using scientific notation at any time in this report, use "E" notation (i.e. 1.5E-3) Stock solution concentration: 2.5 g/L Table view List view Table 4. Standards preparation for 10 mL sample Standard Standard Standard Standard Standard Standard 1 2 3 4 5 6 Patient conc. (g/L) 20.0 30.0 40.0 50.0 60.0 70.0 Actual conc. of standard (g/L) Volume albumin stock solution (mL) Volume of stock NaCl solution (mL) Creating Standards Data Entry Enter the stock solution concentration (g/L) 2.5 Table 1. Standards preparation Standard Standard Standard Standard Standard Standard 1 2 3 4 5 6 Patient conc. (g/L) 20.0 30.0 40.0 50.0 60.0 70.0 Part I: Calibration Curve Data Entry 0.50 Volume of standard solution added to each cuvette Volume of BCG solution added to each cuvette 1.00 Table 2. Standard curve absorbance data Concentration Absorbance Cuvette 1 20.0 0.385 Cuvette 2 30.0 0.407 Cuvette 3 40.0 0.645 Cuvette 4 50.0 0.675 Cuvette 5 60.0 0.873 Cuvette 6 70.0 0.914 Part III. Spectrophotometric Analysis of Patient Samples Data Entry 0.50 Volume of patient sample added to the cuvette Volume of BCG added to the cuvette 1.00 Table 3. Patient samples absorbance and concentration Patient ID Absorbance Patient 1 0.405 Patient 2 G 0.810 (4.5pts) Creating Standards Using the stock solution given, enter the volumes used to prepare the six standard solutions of albumin in NaCl solution to represent 40-fold dilutions of typical "patient" concentrations from 20 to 70 g/L. For each standard the target "patient" concentration is given, you need to record the actual concentration of the standard, the volume of the stock solution used to make the standard, and the volume of NaCl solution used to make the standard. We are assuming you are making 10 mL of each standard solution. NOTE: If you ever need to write a number using scientific notation at any time in this report, use "E" notation (i.e. 1.5E-3) Stock solution concentration: 2.5 g/L Table view List view Table 4. Standards preparation for 10 mL sample Standard Standard Standard Standard Standard Standard 1 2 3 4 5 6 Patient conc. (g/L) 20.0 30.0 40.0 50.0 60.0 70.0 Actual conc. of standard (g/L) Volume albumin stock Using the stock solution given, enter the volumes used to prepare the six standard solutions of albumin in NaCl solution to represent 40-fold dilutions of typical "patient" concentrations from 20 to 70 g/L. For each standard the target "patient" concentration is given, you need to record the actual concentration of the standard, the volume of the stock solution used to make the standard, and the volume of NaCl solution used to make the standard. We are assuming you are making 10 mL of each standard solution. NOTE: If you ever need to write a number using scientific notation at any time in this report, use "E" notation (i.e. 1.5E-3) Stock solution concentration: 2.5 g/L Table view List view Table 4. Standards preparation for 10 mL sample Standard Standard Standard Standard Standard Standard 1 2 3 4 5 6 Patient conc. (g/L) 20.0 30.0 40.0 50.0 60.0 70.0 Actual conc. of standard (g/L) Volume albumin stock solution (mL) Volume of stock NaCl solution (mL) Creating Standards Data Entry Enter the stock solution concentration (g/L) 2.5 Table 1. Standards preparation Standard Standard Standard Standard Standard Standard 1 2 3 4 5 6 Patient conc. (g/L) 20.0 30.0 40.0 50.0 60.0 70.0 Part I: Calibration Curve Data Entry 0.50 Volume of standard solution added to each cuvette Volume of BCG solution added to each cuvette 1.00 Table 2. Standard curve absorbance data Concentration Absorbance Cuvette 1 20.0 0.385 Cuvette 2 30.0 0.407 Cuvette 3 40.0 0.645 Cuvette 4 50.0 0.675 Cuvette 5 60.0 0.873 Cuvette 6 70.0 0.914 Part III. Spectrophotometric Analysis of Patient Samples Data Entry 0.50 Volume of patient sample added to the cuvette Volume of BCG added to the cuvette 1.00 Table 3. Patient samples absorbance and concentration Patient ID Absorbance Patient 1 0.405 Patient 2 G 0.810 (4.5pts) Creating Standards Using the stock solution given, enter the volumes used to prepare the six standard solutions of albumin in NaCl solution to represent 40-fold dilutions of typical "patient" concentrations from 20 to 70 g/L. For each standard the target "patient" concentration is given, you need to record the actual concentration of the standard, the volume of the stock solution used to make the standard, and the volume of NaCl solution used to make the standard. We are assuming you are making 10 mL of each standard solution. NOTE: If you ever need to write a number using scientific notation at any time in this report, use "E" notation (i.e. 1.5E-3) Stock solution concentration: 2.5 g/L Table view List view Table 4. Standards preparation for 10 mL sample Standard Standard Standard Standard Standard Standard 1 2 3 4 5 6 Patient conc. (g/L) 20.0 30.0 40.0 50.0 60.0 70.0 Actual conc. of standard (g/L) Volume albumin stock
