I need help with all of B; #1-5

B. Understanding Concentrations and Measuring Volumes 1. Concentrations of a solution are most commonly expressed as a. molarity b. g/mL 2. Describe how to prepare 100 mLs of 1X PBS from a stock of 10X PBS. 3. How much agarose should be added to 40 mL of buffer to make a 1% gel. Show your work. Name: NEUR 2700 Methods in Neuroscience Pre-Lab 2: Making Solutions 4. You have been provided 100 g of an antibody. a. Demonstrate how you would prepare a stock solution of 1 mg/mL. b. Demonstrate how you would prepare 1 mL working solution of 3 ug/mL from your 1 mg/ml stock. 4. You have been provided 100 ug of an antibody. a. Demonstrate how you would prepare a stock solution of 1 mg/mL. b. Demonstrate how you would prepare 1 mL working solution of 3 ug/mL from your 1 mg/ml stock. 5. You have been provided with 100 ul of antibody that has a concentration of 1 mg/mL. a. Demonstrate how you would prepare a 1 mL working solution at 1:200. b. What is the final concentration of your working solution in mg/mL? B. Understanding Concentrations and Measuring Volumes 1. Concentrations of a solution are most commonly expressed as a. molarity b. g/mL 2. Describe how to prepare 100 mLs of 1X PBS from a stock of 10X PBS. 3. How much agarose should be added to 40 mL of buffer to make a 1% gel. Show your work. Name: NEUR 2700 Methods in Neuroscience Pre-Lab 2: Making Solutions 4. You have been provided 100 g of an antibody. a. Demonstrate how you would prepare a stock solution of 1 mg/mL. b. Demonstrate how you would prepare 1 mL working solution of 3 ug/mL from your 1 mg/ml stock. 4. You have been provided 100 ug of an antibody. a. Demonstrate how you would prepare a stock solution of 1 mg/mL. b. Demonstrate how you would prepare 1 mL working solution of 3 ug/mL from your 1 mg/ml stock. 5. You have been provided with 100 ul of antibody that has a concentration of 1 mg/mL. a. Demonstrate how you would prepare a 1 mL working solution at 1:200. b. What is the final concentration of your working solution in mg/mL