I need to answer this question please!!

In an antibiotic trial, there is a difference in mean bacterial load between treatment and control. Why is this difference not enough to conclude that there is an effect of treatment? What else needs to be factored in before a t-test can be performed and the p-value determined?

s. In an antibiotic trial, there is a difference in mean bacterial load between treatment and control. Why is this difference not enough to conclude that there is an effect of treatment? What else needs to be factored in before a t-test can be performed and p-value determined? 9. In the same antibiotic trial, what do you need to know to determine if the treatment is biologically significant