If the culture volume loading (working volume) is about 80% of the total fermenter tank volume, design the fermenter tank to produce 10kg purified EPO/year step by step by answering the followings:

1)What is the total EPO (kg) required from fermentation to produce 10 kg purified EPO by purification?

2)What is the total fermentation volume/year to produce 10 kg purified EPO by batch and by chemostat individually?

3)If batch mode is operated, how many batches can be operated per year? What is the fermentation volume per batch?

4)If chemostat continuous mode is operated by continuous feeding and continuous harvesting, What is the culture volume in the fermenter in chemostat continuous fermentation to product 10kg purified EPO? What is the fermenter tank volume applied in chemostat?

5)By comparing batch and continuous chemostat fermentation fermenter volume and design, what would you recommend regarding the selection of operation mode of upstream process? Why?