In a study, the researchers tested the effects of glycerol-enhanced hyperhydration (GEH) on endurance in cycling performance. Urine output volumes were measured on 21 subjects in the experiment group (glycerol) with the mean of 1124ml and in 25 subjects in the control group (placebo) with the mean of 1465ml. To determine whether or not the endurance in cycling performance of the two groups are significantly different, an analyst would most likely use:

		One sample t-test
		ANOVA
		Two-sample t-test
		Dependent t-test