In the scope of term project, each group will calculate major process parameters. The design will include the detailed process calculations, operational costs (based on only mixing), and justifications (or assumptions) for the calculations. The report will include the following sections: - Cover Page - Content page - Executive summary of penicillin production - Brief description of process and assumptions - Detailed process calculations - Reference list (All references listed here will be referred in the report) A 20 m3 working volume of bioreactor is used for producing penicillin. The yield of biomass from substrate is 0.06 g.g-1; YPX is 7.7 g.g-1. The maintenance coefficient is 2.2 g.g-1.h-1; the specific rate of product formation due to maintenance is 1.1 h-1 . 2 kg bacteria are inoculated into 20 m3 of medium. Given data: Dtank=2.4m Impeller speed= 90 rpm Number of blades= 8; flat, turbine types of blade Broth viscosity= 1 mPa.s=1 cP = 1.2x103 kg/m3 Aeration rate= 1 vvm Ratio of gassed to ungassed power Pg/P=0.4 Driving force for OTR= 6x10-3 kg/m3 Specific O2 uptake= 0.65 mmol O2/kg cell Specific sugar consumption rate of cells= 1 kg/kg cell.h max= 0.5 h-1

Calculations to do: 1. Draw the batch reactor systems according to height/weight and where impellers located. Also, show required bioreactor components for the antibiotic production. 2. What would be the sugar concentration (So) you choose if oxygen transfer rate is not the limiting reactant? 3. Define kLa. 4. Determine batch reaction time for producing 100 kg antibotic and define the number of batch per year. (Assume down time=30 h) 5. How much does it cost to mix such a reactor per year?