Is my description of synthiese correct? and what is the peptide washed with? I need a clear answer. Can you determine it on a piece of paper

Can u also draw the peptide???????

To synthesize the peptide H-Leu-Ala-His-Tyr-Asn-Lys-NH2 using Fmoc-based solid-phase synthesis, the following steps will be used:

The linker (4-methoxybenzhydrylamine) is attached to the resin, after which the linker is anchored.

Next, the N-terminus is protected with Fmoc and the side chains of His, Tyr, and Asn are protected with DCB, CT, and Trt, respectively.

N,N'-dicyclohexylcarbodiimide (DCC) is then used as the coupling reagent to add each amino acid building block (Leu, Ala, His, Tyr, Asn, and Lys) to the growing peptide chain.

The process is repeated with deprotection, coupling, and washing for each subsequent amino acid in the peptide sequence.

The final peptide product is obtained by removing the protecting groups, cleaving it from the resin, and purifying it.

The peptide H-Leu-Ala-His-Tyr-Asn-Lys-NH2 is an N-terminal amide, which is why a linker that provides peptide amides is needed. The peptide is synthesized using a polystyrene resin, and a linker is required to attach the first amino acid to the resin. For this peptide, an MBHA linker can be used. It has the ability to bind Fmoc-protected amino acids to the resin and can also be easily released during deprotection.

In Fmoc-based solid-phase synthesis, the side chains of the amino acids are protected with specific groups. The peptide H-Leu-Ala-His-Tyr-Asn-Lys-NH2 is protected with the following side chain protecting groups: Leucine: t-butyl (tBu) Histidine: tert-butyloxycarbonyl (Boc) Tyrosine: tert-butyldimethylsilyl (?) Asparagine: trityl (Trt) Lysine: 2-chlorotrityl (2-ClTrt)

Coupling reagents are used to activate the amino acid for binding to the growing peptide chain. For this synthesis, N,N'-diisopropylcarbodiimide (DIC) and 1-hydroxybenzotriazole (HOBt) will be used as the coupling reagents.

The amino acid building blocks for this synthesis are as follows: Fmoc-Leucine-OH Fmoc-Alanine-OH Fmoc-Histidine(Trt)-OH Fmoc-Tyrosine(?)-OH Fmoc-Asparagine(Trt)-OH Fmoc-Lysine(2-ClTrt)-OH

Provide a detailed plan and explanation on how to synthesize the following peptide by using Fmoc-based solid phase synthesis: H-Leu-Ala-His-Tyr-Asn-Lys-NH2 The written report should include a description of the choice of resin, linker, protecting groups, coupling reagents, amino acid 'building blocks', etc. needed in order to conduct this synthesis