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It is desired to carry out the above sterilization in a fermentor of different size. If the new fermentor contains one fifth of the volume

It is desired to carry out the above sterilization in a fermentor of different size. If the new fermentor contains one fifth of the volume of the original and the times for heating up and cooling down between

100 C

and

121 C

are

15

min and

10

min, respectively. What should be the holding period?

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