It is intended to obtain the product P by hydrolyzing the substrate S using an enzyme (E) immobilized on the smooth support surface without pores.

The values measured by the enzyme reaction rate according to the change in the concentration of the substrate on the surface of the support are shown in the table below.

Substance transfer coefficient of substrate is 0.2 cm/s. Answer the following question.

(1) When the substrate concentration [Sb] in the solution is 10 mmol/L, find the substrate concentration [Ss] in the support bubble.

(2) Under the conditions of (1), the rate of enzyme reaction is either rate-limited or diffusion-limited, and why.

(3) If the area where the enzyme is fixed on the support is 2000cm^2, calculate the rate of substrate decomposition in mmol/min.

(S) mmol/L 0.1 0.3 0.5 0.7 1.5 3 V mmol/cms 0.44 1.09 1.54 1.87 2.61 3.16 3.33 3.59 4 7