1/ You need to count colonies on a plate. The plate was created using a 1/103 dilution of a bacteria culture. You count 50 colonies after plating one ml of diluted sample. How many cells in the original tube?

a.500

b.5000

c.50000

d.0.05000

2/ You are using media that contains 2% w/v agar. In 1L, how much agar do you need to add to get that %?

		2 g
		20 g
		200 g
		cannot answer without the molecular weight

3/ What is not true about the streak for isolation technique?

		isolated colonies will be always found in the 4th quadrant
		You must flame your loop between each quadrant
		You cannot put the lid of the plate on the bench while working.
		The loop must be made orange when flaming it to ensure that it is hot enough.

4/ Which tube uses a needle rather than a loop for inoculation?

		broth
		deep
		slant
		anaerobic