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Lab 1 Analysis of purification using SDS-PAGE You perform an SDS-PAGE gel on your protein purification samples. You observe no bands in the lane for

Lab 1

Analysis of purification using SDS-PAGE

  1. You perform an SDS-PAGE gel on your protein purification samples. You observe no bands in the lane for your elution fraction, but you see a strong ~92 kDa band in the total cell lysate and flow through lanes. What could be a reason for this observation? (think about the purification).

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