Lab 7: Acid & Enzyme Hydrolysis of G-6-P

Student's name & Which experiment did you do?
Partner's name & Which experiment did they do?
Prac day and time
Teaching Associates name
Date

INTRODUCTION:

In your own words provide appropriate background information on the experiment and concisely explain relevant theories. As part of the introduction address the following:

• Background biological context
• What the experiment will address
• The reaction being utilised as part of the experiment and how this can be studied

(5 marks)

AIM:

State the aim/purpose of the experiment (2 marks)

METHOD:

Attach flow diagram illustrating the main aspects to be undertaken in the lab 7 experiment. (10 marks)

RESULTS:

Phosphate standard curve
Standard tubes	1	2	3	4	5	6
Pi (mol)	0	0.1	0.2	0.3	0.4	0.5
0.5 mM phosphate (uL)	0	200	400	600	800	1000
H2O (uL)	1000	800	600	400	200	0
Absorbance at 660 nm	0.123	0.323	0.517	0.692	0.872	1.035
Absorbance (-background)	0	0.2	0.394	0.569	0.749	0.912

Graph: Include a graph of your phosphate standard curve. Should include the following elements; Appropriate Title, correct axis (with units where appropriate), key, appropriate scale, line of best fit (+ equation, R²), and figure legend.

\f